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Lena Smirnova +1 more instructor

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Toxicology 21: Scientific Applications

Johns Hopkins University

About this Course

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This course familiarizes students with the novel concepts being used to revamp regulatory toxicology in response to a breakthrough National Research Council Report “Toxicity Testing in the 21st Century: A Vision and a Strategy.” We present the latest developments in the field of toxicology—the shift from animal testing toward human relevant, high content, high-throughput integrative testing strategies. Active programs from EPA, NIH, and the global scientific community illustrate the dynamics of safety sciences.

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Intermediate Level

Approx. 96 hours to complete

English

Subtitles: French, Portuguese (European), Russian, English, Spanish

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Flexible deadlines

Reset deadlines in accordance to your schedule.

Shareable Certificate

Earn a Certificate upon completion

100% online

Start instantly and learn at your own schedule.

Intermediate Level

Approx. 96 hours to complete

English

Subtitles: French, Portuguese (European), Russian, English, Spanish

Could your company benefit from training employees on in-demand skills?

Try Coursera for Business

Instructors

Instructor rating

4.68/5 (25 Ratings)

Lena Smirnova

Research Associate

Center for Alternatives to Animal Testing

13,717 Learners

2 Courses

Thomas Hartung

Professor

Environmental Health and Engineering

13,717 Learners

2 Courses

Offered by

Johns Hopkins University

The mission of The Johns Hopkins University is to educate its students and cultivate their capacity for life-long learning, to foster independent and original research, and to bring the benefits of discovery to the world.

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Syllabus - What you will learn from this course

Week

Week 1

75 hours to complete

Module 1

This model will give you a broad introduction to the Toxicology 21st century (Tox21c) field and familiarize you with the main document, which summarize the main ideas and principles of Tox21c.

75 hours to complete

10 videos (Total 121 min), 3 readings, 1 quiz

10 videos

Welcome to Module 14m

Course Introduction16m

Introduction49s

Center for Alternatives to Animal Testing4m

Toxicology of the Fifteenth to Twentieth Centuries15m

Toxicology for the Twenty-First Century15m

Historical Perspective17m

The First Report: Toxicity Testing for Assessment of Environmental Agents11m

The Second Report: Toxicity Testing in the 21st Century: A Vision and A Strategy Vision and Its Components22m

The Second Report: Toxicity Testing in the 21st Century: A vision and A Strategy Implementation13m

3 readings

useful links20m

supporting reading material10m

Supporting reading material10m

1 practice exercise

Module 1 Quizs

Week

Week 2

4 hours to complete

Module 2

This module will discuss how ToxCast and Tox21 technologies can be used in practice. The US EPA Endocrine Disruptor Screening Program will be discussed in detail and we will demonstrate how the ToxCast platform replaced in vivo tiered approaches in EDSP and, thus, acelerate the process of . In the second lesson of this module, the development of in silico models for toxicity testing will be discussed.

4 hours to complete

10 videos (Total 119 min), 4 readings, 2 quizzes

10 videos

Welcome to Module 22m

Adverse Outcome Pathways (AOPs)10m

Exposure-Based Chemical Prioritization13m

ER Bioactivity Model16m

US EPA Endocrine Disruptor Screening Program (EDSP)17m

Modeling of Hazard and Organ Manifestation11m

Building a Signature for Vascular Disruption10m

Testing the Vascular Disruption Signature8m

Virtual Tissue Model: Vascular Development12m

Functional Vascular Development Assays16m

4 readings

Supporting Reading Material10m

Useful links10m

Supporting Reading Material10m

Useful links10m

1 practice exercise

Module 2 Quiz30m

Week

Week 3

3 hours to complete

Module 3

This module will familiarize you with the Human Toxome Project run by Johns Hopkins University in collaboration with several university, industry, and research agencies. Omics technologies, their advantages and disadvantages, as well as concepts of Adverse Outcome Pathways (AOP) and Pathways of Toxicity (PoT) will be discussed.

3 hours to complete

10 videos (Total 109 min), 2 readings, 1 quiz

10 videos

Welcome to Module 31m

Mechanistic Toxicology and Pathway Mapping at the Basics of 21st Century Toxicology16m

The Concept of the Human Toxome Project21m

Concept and Tool Devlopment11m

Progress7m

Conclusions and Prospectives8m

Introduction: What Is a Pathway of Toxicity, and Why Do We Need Them?5m

Omics Technologies and High-Throughput Data6m

Interpreting Omics Data11m

Using Network Approaches to Understand Toxicity Mechanisms18m

2 readings

Supporting reading materials10m

Supporting reading material10m

1 practice exercise

Module 3 Quiz30m

Week

Week 4

5 hours to complete

Module 4

The first lesson in this module will explain the concept of read-across, why it is important for regulatory risk assessment, and how it can be implemented by industry in response to REACH legislation. The organotypic cultures lesson will teach you about existing in vitro cell-based models starting from simple monolayer cultures of cell lines and ending with complex combined organ-on-a-chip and human-on-a-chip approaches.

5 hours to complete

18 videos (Total 185 min), 3 readings, 2 quizzes

18 videos

The Need, and Opportunity, for a New Approach to Toxicology, Including Predictive Tools7m

The Concept of Toxicological (Biological) Pathways, e.g., “Adverse Outcome Pathways,” Is the Basis of Predictive Toxicology5m

Introduction to the OECD AOP Framework10m

Building, Evaluating, and Using AOPs in Safety Decisions6m

Online Tools Available to Everyone, Including an Online AOP Course4m

Welcome to Module 42m

Introduction to Read-Across18m

Good Read-Across Practice21m

Supporting Read-Across with Biological Data12m

Creating a Database of Toxicity12m

Creating an Automated Tool for Read-Across9m

Cell Sources for In Vitro Models12m

Monolayer Cultures4m

Advanced Cell Cultures: Microphysiological Systems (MPS)7m

3D Cultures25m

Microfluidics2m

Organ-on-Chip, Body-on-Chip13m

Good Cell Culture Practice7m

3 readings

Supporting reading material10m

Useful links10m

2 practice exercises

Module 4 Quiz30m

Mid-term exam30m

2 hours to complete

Module 5

This module will teach you the role of miRNA and epigenetics in toxicology and environmental health.

2 hours to complete

6 videos (Total 41 min), 1 reading, 1 quiz

Week

Week 5

4 hours to complete

Module 6

In this module you will learn about important component of in vitro toxicology such as in vitro to in vivo extrapolation (IVIVE) and physiologically based biokinetic modeling. You will learn why it is important to consider biokinetics in vitro and see some example of biokinetics modeling in vitro. This lesson will also give you a biometry prospective of in vitro toxicology. You will learn which methods can be used to analyze big data.

4 hours to complete

8 videos (Total 106 min), 3 readings, 2 quizzes

8 videos

Welcome to Module2m

Physiologically Based Biokinetic Modeling10m

Application of QIVIVE to in Vitro Testing14m

Challenges for Conducting QIVIVE14m

Quantitative in Vitro to in Vivo Extrapolation25m

Definition of Biometry/Biostatistics and Revisiting Some Statistical Basics17m

Types of Tox21 Data and Data Mining of External Data Sources8m

Bioinformatics Tools for the Interpretation of Tox21 Data13m

3 readings

Supporting reading material10m

Useful links10m

1 practice exercise

Module 6 Quiz30m

Week

Week 6

4 hours to complete

Module 7

In this lesson you will learn about integrated testing strategies and how they differ from individual tests or test batteries. Also a wrap-up lesson "beyond chemical" will summaries your knowledge from this course and give a out-look for further directions in the field of alternatives to animal testing.

4 hours to complete

11 videos (Total 143 min), 2 readings, 2 quizzes

11 videos

Welcome to Module 73m

Why Integrated Testing Strategies?5m

What is an Integrated Testing Strategy?16m

How to Compose Integrated Testing Strategies36m

Strategies for Integrated Testing Strategies6m

Quality Assurance of integrated Testing Strategies7m

Legal Background13m

Economic Background23m

Tox-21c for Drug Safety12m

Green Toxicology as Non-regulatory Tox-21c Approaches12m

Conclusions and Prospects7m

2 readings

Supporting reading material10m

2 practice exercises

Module 7 Quiz30m

Final Exam30m

Reviews

4.7

22 reviews

5 stars
79.72%
4 stars
12.16%
3 stars
5.40%
1 star
2.70%

TOP REVIEWS FROM TOXICOLOGY 21: SCIENTIFIC APPLICATIONS

by OANov 19, 2018

Awesome course! Highly essential information for all toxicologists!

by NKDec 26, 2018

A very well-taught course with lots of useful information. I highly recommend it.

by SMay 3, 2018

Very nicely designed course. The instructors offer an excellent and easy to understand overview of a range of promising Tox21 tools and approaches using good examples and illustrations.

by MMNov 29, 2020

Very nice and the teachers explanation are clear . It’s very interesting, specially if you work in the research-lab section

View all reviews

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Toxicology 21: Scientific Applications

Toxicology 21: Scientific Applications

About this Course